The
system used to culture viruses depends on?
•
Availability
•
laboratory facilities
•
personnel and viral agent being studied
What is
the most common tissue used in cell culture to grow viruses in?
•
Kidney cells
What is
contact inhibition phenomen and when does it occur?
•
As the cells multiply and begin to come into contact
with one another, there is a feedback effect cause them to stop growing.
•
It takes about 10 - 4 days for this to occur.
•
Contact inhibition will cause the production of a
confluent monolayer of cells in a cell culture.
What are
the two types of media used to produce confluent cell sheets to be used for
viral research?
1.
Growth media - used when the cell sheet is forming
2. Maintenance medium - used when the cell
sheets are confluent and they are to be infected with the virus.
The
system used to culture viruses depends on?
•
Availability
•
laboratory facilities
•
personnel and viral agent being studied
What is
the most common tissue used in cell culture to grow viruses in?
•
Kidney cells
What is
contact inhibition and when does it occur?
•
As the cells multiply and begin to come into contact
with one another, there is a feedback effect cause them to stop growing.
•
It takes about 10 - 4 days for this to occur.
•
Contact inhibition will cause the production of a
confluent monolayer of cells in a cell culture.
What are
the two types of media used to produce confluent cell sheets to be used for
viral research?
1.
Growth media - used when the cell sheet is forming
2. Maintenance medium - used when the cell
sheets are confluent and they are to be infected with the virus.
True or
False: when the monolayer is
established, the growth media is removed.
•
T - growth media is removed and replaced by maintenance
media
What are
types of cells used in T.C, their origins, and their no. of chromosomes?
·
Primary cell culture: Plant, animal, insect or human Diploid
(2n)
·
Diploid cell strain: Remnant
of primary cell culture Diploid (2n)
·
Established cell line: tumor
cells or mutagenesed normal cells Heteroploid (>2n)
Define
primary cell culture.
•
Cultures established initially from tissue taken
directly from animals,
•
e.g. fetal organs
or tissues. They contain several cell
types.
Define
Diploid cell strain.
•
Sereially propagated primary cell lines that are capable
of undergoing many more divisions.
•
They retain their
diploid chromosome number.
Define
Continuous cell line.
•
Cells of a single type that are capable of indefinite
propagation in vitro.
•
An immortal cell
line originating from cancers, or by spontaneous transformation of a diploid
cell strain.
•
Do not resemble parent cell because of mutations and are
often aneuploid (abnormal) in chromosome number.
What is anchorage
phenomenon and when does it occur?
Mean the
ability of cells to settle down and attached firmly to T. C. vessels
What are
the factors affecting anchorage phenomenon ?
1-Type of
the cell (adherent).
2- Viability
of the cell (viable).
3- Culture
vessel surface charge (+ve).
4-
Incubation temperature (37 oC).
5- Component
of the media: as Calcium and Fibrinonectin
True or
False:
MDBK – BHK and
Vero (African green monkey kidney) cells are anchored cells while,
Myeloma
cells - SF-9 cells and High Five cells are non anchored cells (T)
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