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Friday, February 28, 2014

Question @ Answer Practical - Media

True or False. 
  • ·         Horse serum, Newborn calf serum, Fetal calf serum, Lactalbumin hydrolysate are of nature media - T
  • ·         Minimal essential medium (MEM), Rosen well Park Memorial Institute ( RPMI) are of nature media – F ( artificial)
  • ·         Any virus can be grown in any of the previously mentioned media.
     F.  There isn’t one specific media that can grow every virus because,
     there isn’t one that has every possible adhesion molecule available for every virus to attach to it.
  • Phenol red is added to the media as a______

          pH indictor
What are types of media to their nutritive value & uses:
  • ·         Balanced salt solution for few hours
  • ·         Maintenance media for few days
  • ·         Growth media for long period

Maintenance media contains ___
  • ·         MEM +1-2% FCS

Balanced salt media contains ___
  • ·         saline Or PBS

Growth media contains ___
  • ·         MEM +5-10% FCS

____ is used to supply additional growth factors required by most cells.

          (FCS) Fetal calf serum (5 - 10%)

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