Question & Answer Practicle - Vessels

True or False. 

•          Hot air oven with 2 hours at 200ºC used for sterilization of metal and glass - T

•          Autoclave with 20-30 minutes at 121ºC under high pressure  used for sterilization of:

            plastic, rubber,metal, glass and fluid not contain protein.T

•          UV chamberwith 2 hours of exposure used for sterilization of plastic and rubber.T

•          In Filtration system:

•           membrane filter of 0.22 μm used for sterilization of all fluids and reagents that,

             contain proteinous material or components damaged by heat

• •          Water bath used during manipulation of cells.F ( before)
• •          Horizontal Laminar air flow cabinet (workstation) used for Dangerous Viruses – F (Culture of Cells only )
• •          Vertical Laminar air flow cabinet (workstation) used for non dangerous Viruses - T
• •          Biohazard Laminar air flow cabinet (workstation) used for Culture of Cells only – F (Dangerous Viruses )
• •          Ordinary incubator and CO₂ regulated are equipment used after manipulation of cells.T
• •          Gas exchange CO2 incubator are 2 methods of closed system of ventilation – F (open)
• •          Ordinary Incubator is one method of closed system of ventilation – T
• •          TC plates, Ventilated prescriptions, Glass, Plastic Petridishes used in open system of ventilation -T
• •          Roux, Closed prescription, TC tubes, CCSC used in open system of ventilation – F ( Closed)
• •          Phosphate Buffered Saline (PBS) is one of buffer used in tissue culture. - T

What are the equipment during manipulation of cells

• •          Laminar air flow cabinet  (workstation)
• •          Inverted microscope
• •          Automatic pipetting aid
• •          Magnetic stirrer
• •          Automatic pipettes