How are
viruses cultivated in the lab?
•
In cultured cell media
•
In embryonated hen’s egg
•
in laboratory animal
What are
the different uses of Tissue Culture
•
1-Isolation of different viruses.
•
2- Characterization and identification of viruses.
•
3- Propagation of viruses for antigen and vaccine
production.
•
4- Titration of viruses.
•
5- Host for SNT, cell ELISA and FAT.
•
6- Studying the viral cytopathogenecity.
•
7- Studying the growth curve and multiplication cycle of
different viruses.
•
8- Preparation of monoclonal antibodies to a wide
diversity of viral proteins.
•
9- Invitro expression of viral proteins.
•
10- Testing the antiviral activity of various chemicals
and drugs.
What are
the advantages of Tissue Culture
•
Support growth of most viruses.
•
2- Economic.
•
3- No immune system.
•
4- Low level of extraneous proteins so easy virus
purification.
What are
the disadvantages of Tissue Culture
•
1-Latent infection.
•
2- Difficult.
•
3- Costs for establishing a tissue culture laboratory
(Expensive).
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