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Friday, February 28, 2014

Question & Answer -2 General Virology

Viral enzymes are required for?
          Interaction with receptors on host cell
          transcribing the viral genome into mRNA
          involved in copying virion RNA into DNA
          Required for nucleic acid replication
What is the viral enzyme neuraminidase required for?
          Receptor-destroying enzyme
          allows viruses to detach or elute from cells
In what viruses is neuraminidase found?
          Orthomyxoviruses
          Paramyxoviruses
What does the viral enzyme RNA-dependent DNA polymerase do?
          It is used for reverse transcription
          found in
        retroviruses (RNA virus)
        hepadnaviruses (DNA virus)
If a virus has a positive (+) transcriptase, it cannot replicate in the host cytoplasm.
          F, If it has a (+) transcriptase it means that it is providing its own transcriptase and therefore can divide in the cytoplasm. 
All DNA viruses are double stranded except?
          Parvoviruses
          Circoviruses
All RNA viruses are single stranded except?
          Reoviruses
          birnaviruses
An example of the RNA viruses that copy their genome into DNA is:
          HIV
If an virus contains a positive strand RNA, it acts directly as _____.
          mRNA
What is a prion?
          Small, proteinaceous, infectious particle that is highly resistant to heat, formalin, and chloroform.
What is the suffix for family?
          -viridae
What is the suffix for subfamily?
          -virinae
What is the suffix for genera?
          -virus
Viruses are divided into families based on?
          Size
          symmetry
          substructure
          type of nucleic acid genome (RNA or DNA)
          form of genome
        Single stranded or Double stranded
        segmented or nonsegmented
         cyclic or linear
         haploid or diploid)
          mode of replication


Viruses are divided into genera and species based on?
          Host species
          pathogenesis
          nucleic acid homology
          antigenicity
Name 3 important characteristics of the Family Adenoviridae.
          Possess filaments which project from vertex capsomeres - help attach to cell
          Form basophilic (blue) intranuclear inclusion bodies
          Associated with latent infections
The genus Parvovirus depends on _____ for replication and grows best in _____ and forms ____ in cell.
          Cellular enzymes
          rapidly dividing cells
          acidophilic (red) intranuclear inclusion bodies when stained with H & E.
Two family of viruses which create intracytoplasmic inclulsion bodies are _____ and ____ and is a large virion.
          Family Iridoviridae
          Family Asfarviridae
The viruses that belong to the Family herpesviridae mature by ________ and therefore are ______.
          Budding thruogh the nuclear membrane
          Enveloped
Herpesvirus form ______ in the cell nucleus.
          Acidophilic (type A) intranuclear inclusion bodies
_____ is a family of viruses that have an affinity for the liver.
          Hepadnaviridae
What is pathoneumonic of the viral family Hepadnariridae?
          They are the only DNA virus with reverse transcriptase. 
What does reverse transcriptase do for a DNA virus?
          In an DNA virus, when the virus genome is first translated, it is translated into RNA.
          The reverse transcriptase is then used to translated back into a DNA genome.
______ This family of viruses is the smallest known virus of vertebrates and plants.
          Circoviridae
What type of genome does the Family circoviridae have and where does replication occur?
          Single stranded DNA genome
          Replication occurs in the nucleus of cycling cells
What is the genome of the family Togaviridae?
          (+) sense RNA with envelope
          Icosahedral nucleocapsid
          ss RNA
What type of genome does the family Reoviridae have?
          (+) sense, ds RNA, segmented
Reoviridae has a  unique capsid.  Describe it.
          It possess a two-layered capsid
          The capsomeres have holes
Describe the inclusion bodies that Reoviridae produces.
          Acidophilic perinuclear (c-shaped) inclusion bodies
What type of genomes does the family Coronaviridae have?
          (+) sense, ss RNA, enveloped
What is unique about the envelope that is found around the capsid in the family Coronaviridae?
          It possess unique club-shaped peplomeres projecting from the envelope
Most members of the Bunyaviridae are transmitted by _____.
          ARTHROPODS
What type of genome does the family Bunyaviridae have?
          (+) sense, ss RNA, enveloped
          carries its own transcriptase
The influenza virus falls into this family: ____
          Orthomyxoviridae
The Orthomyxoviridae have what type of genome?
          (_) sense RNA; segmented (7-8 segments); enveloped
          carries its own transcriptase
The Orthomyxoviridae have glycoprotein peplomeres consisting of ____ and _____.
          Hemagglutinin and
          neuraminidase
Where does replication of Orthyomyxoviridae take place?
          In the nucleus and cytoplasm of the host cell
Why is dual infection in the Orthyomyxoviridae important?
          It provides the virus with the possibility of genetic reassortment creating new flu viruses.
This family have pleomorphic virons:_____
          Paramyxoviridae
True or False.  The Paramyxoviridae form both intracytoplasmic and intranuclear inclusion bodies.
·         T
True or False.  All Paramyxoviridae possess both hemaglutinin and/or neuraminidase.
          F.  Only some of them possess these.
True or False.  Canine distemper virus is a memmber of the Retroviridae family.
          F.  It is a member of the Paramyxoviridae family
The type of inclusion bodies that the rabies virus produces are called _____
          Negri bodies (red intracytoplasmic inclusion bodies)
The virion in this family resembles a bullet:_____
          Rhabdorviridae
What type of genome does the family Retroviridae have?
          (+) sense , ss, RNA; enveloped
          carries its own transcriptase
The family Retroviridae is unique in that it prosses a ____ and,
 this family possess all RNA ____ viruses and these viruses cause _____________.
          Reverse transcriptase
          oncogenic
          permanent infections with periods of latency
True or False.  All oncogenic viruses are classified in the family Retroviridae BUT
, not all Retroviridae virions are oncogenic.
        T
____ is and important human virion and ____ is an important feline virion which both fall into the family Retroviridae.
          HIV
          FIV
The Birnaviridae family has a ______ genome.
          Ds RNA

          possess own transcriptase

What does a segmented genome allow?
          Genetic reassortment


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