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Thursday, February 27, 2014

Question & Answer-1 General Virology

What are the differences between pseudotypes and Pseudovirions?
Pseudotypes:
          This happens during mixed viral infections
          During replication in co-infected cells,
          the genome of one virus may become encapsidated in the heterologous protein coat encoded by the second virus
Pseudovirions
·         During viral replication the capsid sometimes encloses host nucleic acid rather than viral nucleic acid. 
·         Such particles look like ordinary virus particles when observed by EM, but do not replicate.

All animal viruses helical nucleocapsids are wound into a coil and enclosed within a lipoprotein envelope.
What is the envelope composed of?
          Matrix protein - viral specific
          lipid bilayer - from host cell
          glycoprotein coat virus specific
What is important about the maxtrix protein?
          It is the inner layer of the envelope
          It is viral-specified
          serves as a recognition site for the nucleocapsid at the plasma membrane
          provides added rigidity to the envelope
What is important about the lipid bilayer?
          Comes from the host cell
          it is the middle layer of the envelope


What is important in the glycoprotein layer?
          Outer layer of envelope
          contains projecting peplomers which are virus-specified
          important immunogens
Where do viral carbohydrates come from and what are they used for?
          They are host cell derived
          They are found associated with virus specified proteins
          They form glycoprotein outer layer of the envelope
How does the envelope affect the infectivity of most enveloped viruses?
          The envelope contains attachment sites.
          The integrity of the envelope is essential for infectivity in most viruses
Which virus possesses a viral envelope that is not necessary for infectivity?

          Poxvirus

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